Fish Analysis, Chromosome-Specific Interphase

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FISH ANALYSIS, CHROMOSOME-SPECIFIC INTERPHASE

TURNAROUND TIME: 7 to 10 days

 
TESTING METHODOLOGY:
Fluorescence in situ hybridization
 
SPECIMEN REQUIREMENTS:
  • Collect: 3-5 mL peripheral blood in sodium heparin (green) for children and adults; 1-2 mL peripheral blood in sodium heparin (green) for newborns
  • Min. Collection: 1 mL for newborns; 2 mL for children and adults
  • Transport: peripheral blood in sodium heparin (green) at 20-25°C
  • Stability: Ambient: 24 hours; Refrigerated: 72 hours; Frozen: unacceptable
  • Unacceptable Conditions: Frozen or clotted specimens; specimens in anticoagulants other than sodium heparin

A Cytogenetics Laboratory Test Requisition must accompany the specimen. Contact the Cytogenetics Laboratory at 918-502-1722 to obtain further information.

INTERPRETIVE DATA:
 
Test Summary: Test can detect trisomies of a specific chromosome or duplications of a chromosomal region.
 
 
Methods: A dual-color FISH analysis performed on interphase cells using probes for a specific chromosome locus and a control locus; analysis of 10 metaphase cells and 100 interphase cells
 
Indications for Use:
  • Patients suspected of having a constitutional chromosome duplication or trisomy based on clinical features
  • Confirmation of a chromosome abnormality suspected on routine or high resolution chromosome analysis
ADDITIONAL RESOURCES:
Genetics Home Reference: https://medlineplus.gov/genetics/