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Fish Analysis, Chromosome 8 Enumeration

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FISH ANALYSIS, CHROMOSOME 8 ENUMERATION



Pseudonyms: Trisomy 8, Myelodysplastic syndrome, MDS, Acute Myeloid Leukemia, AML, Chronic Myeloid Leukemia, CML, Myeloproliferative disorders, MPD, Lymphoproliferative disorders, ALL

TURNAROUND TIME: 7 to 10 days
 
TESTING METHODOLOGY: Fluorescence in situ hybridization (FISH) Analysis
 
SPECIMEN REQUIREMENTS:
  • Collect: 2 mL bone marrow aspirate in sodium heparin (green); 5 mL peripheral blood in sodium heparin (green) also acceptable
  • Minimum Collection: 1 mL bone marrow or 2 mL peripheral blood
  • Transport: bone marrow or peripheral blood in sodium heparin (green) at 20-25°C; specimen should arrive in the laboratory within 24 hours of collection
  • Stability: Ambient: 24 hours; Refrigerated: 24 hours; Frozen: unacceptable
  • Unacceptable Conditions: Frozen or clotted specimens; paraffin-embedded specimens; specimens in anticoagulants other than sodium heparin
A Cytogenetics Laboratory Test Requisition must accompany the specimen. Contact the Cytogenetics Laboratory at 918-502-1722 to obtain further information.
 
INTERPRETATIVE DATA:
 
Test Summary: Test can detect trisomy 8 observed in some patients with myelodysplastic syndrome, AML, CML, myeloproliferative disorders and lymphoproliferative disorders; for diagnostic specimens, it is recommended that test be performed with chromosome analysis
 
 
Methods: A single-color FISH analysis performed on interphase nuclei using probe for the D8Z1 which can detect gain of chromosome 8; analysis of 200 interphase cells; analysis of 500 interphase cells for minimal residual disease assessment
 
Interpretation: A positive result is reported when the percent of cells with an abnormality exceeds the normal reference range for the probe. The detection of an abnormal clone indicates a diagnosis of a neoplastic disorder with trisomy 8. A negative result indicates no abnormality was observed but does not rule out the presence of a neoplastic disorder.
 
FDA Approval: This test is approved by the FDA for in vitro diagnostic testing
 
Indications for Use:
  • Patients with trisomy 8; establishing the percentage of neoplastic cells at diagnosis
  • Identifying patients with a neoplastic disorder and trisomy 8
  • Monitoring the chromosome 8 abnormality during treatment

Additional Resources:


http://atlasgeneticsoncology.org/Anomalies/tri8ID1017.html