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25 - HYDROXYVITAMIN D ( D2 and D3 ) LEVELS BY LCMSMS
TURNAROUND TIME: 2 - 3 days
PERFORMED: Monday - Friday
TESTING METHODOLOGY: High Performance Liquid Chromatography Triple Quadrupole Mass Spectrometer (LC/MSMS).
SPECIMEN REQUIREMENTS:
- Collect: 5 ml Gold (serum) or Red top tube. Separate serum (min volume required is 0.5 ml)
- Min. Collection: 1.0 ml whole blood
- Transport: Refrigerated
- Stability: After serum separation. Ambient: 24 hours; Refrigerated: 1 week. Frozen: 1 month
- Unacceptable Conditions: Room Temperature specimens older than 24 hours.
* There are no established reference ranges for 25-Hydroxy Vitamin D2 and D3 alone.
REFERENCE INTERVAL:
Normal ranges are based upon literature values and expert opinion (Table 1) until population specific values are attained.
25-Hydroxy Vitamin D Total (D2 and D3):
|
< 10 ng/ml
|
Deficiency
|
|
10 – 30 ng/ml
|
Insufficiency
|
|
30 – 100 ng/ml
|
Sufficiency
|
|
>100 ng/ml
|
Possible Toxicity
|
INTERPRETATIVE DATA:
25-Hydroxy Vitamin D Total (D2 and D3) :
|
< 10 ng/ml
|
Deficiency
|
|
10 – 30 ng/ml
|
Insufficiency
|
|
30 – 100 ng/ml
|
Sufficiency
|
|
>100 ng/ml
|
Possible Toxicity
|
Vitamin D helps the body absorb calcium, which is needed for bone growth and health. A lack of vitamin D can lead to bone diseases such as osteoporosis or rickets. Vitamin D also has a role in the nerve, muscle, and immune systems.
There are three sources of Vitamin D : through the skin after sunlight exposure, diet, and from supplements.
Analytic Sensitivity: >99%
Test Limitations: This methodology is not valid for children less than 1 year old. Significant concentrations of C-3 epimers of 25-OH vitamin D2 and D3 are commonly found in infants. This can lead to overestimation of total 25-OHD levels. Measurement of total 25-OHD in children less than 1 year of age will therefore not be performed by LC/MSMS at this facility. These specimens will be outsourced for analysis.
INDICATIONS FOR USE:
The intended purpose of this procedure is to measure total
Vitamin D levels in serum using Liquid chromatography-tandem mass spectrometry (LC/MSMS).
ADDITIONAL RESOURCES:
MedLine Plus - Vitamin D Deficiency:
MedLine Plus - Vitamin D Testing: http://www.nlm.nih.gov/medlineplus/ency/article/003569.htm